Video: DNA Ligation Reactions: Principle, Procedure, and Applications
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Video: DNA Ligation Reactions: Principle, Procedure, and Applications
198.6K Views. In molecular biology, ligation refers to the joining of two DNA fragments through the formation of a phosphodiester bond. An enzyme known as a ligase catalyzes the ligation reaction. In the cell, ligases repair single and double strand breaks that occur during DNA replication. In the laboratory, DNA ligase is used during molecular ...
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Saksham Bhatia on Instagram: "DNA ligase is an essential enzyme that joins breaks in the DNA backbone by forming phosphodiester bonds between adjacent nucleotides. It is crucial for maintaining the continuity and stability of DNA molecules. 🔬 Function DNA ligase seals nicks (gaps) that occur between the 3'-hydroxyl (–OH) end of one nucleotide and the 5'-phosphate (–PO₄) end of another. This process is vital in: DNA replication – joins Okazaki fragments on the lagging strand. DNA repair – fixes
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Saksham Bhatia on Instagram: "DNA ligase is an essential enzyme that joins breaks in the DNA backbone by forming phosphodiester bonds between adjacent nucleotides. It is crucial for maintaining the continuity and stability of DNA molecules. 🔬 Function DNA ligase seals nicks (gaps) that occur between the 3'-hydroxyl (–OH) end of one nucleotide and the 5'-phosphate (–PO₄) end of another. This process is vital in: DNA replication – joins Okazaki fragments on the lagging strand. DNA repair – fixes
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